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M9650948.TXT
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1996-03-30
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Document 0948
DOCN M9650948
TI Recovery of mycobacteria from blood in mycobacteria growth indicator
tube and Lowenstein-Jensen slant after lysis-centrifugation.
DT 9505
AU Hanna BA; Walters SB; Bonk SJ; Tick LJ; Department of Pathology, New
York University School of Medicine,; New York 10016, USA.
SO J Clin Microbiol. 1995 Dec;33(12):3315-6. Unique Identifier : AIDSLINE
MED/96156151
AB Five hundred five blood samples for culture were processed in the
Isolator lysis-centrifugation system and were then inoculated into a
Mycobacteria Growth Indicator Tube (MGIT) and onto a Lowenstein-Jensen
(L-J) slant. Forty-nine isolates of Mycobacterium avium complex and
three isolates of Mycobacterium tuberculosis were recovered from 50 of
the blood culture specimens. Forty-five isolates from 43 specimens were
recovered in the MGIT, with a mean time to detection of 21 days.
Forty-one isolates from 40 specimens were recovered in the L-J slants,
and the mean time to detection was 36 days. Nine specimens were positive
in the MGIT alone, while seven specimens were positive only in L-J
medium.
DE AIDS-Related Opportunistic Infections/COMPLICATIONS/DIAGNOSIS/
MICROBIOLOGY *Bacteriological Techniques Blood/*MICROBIOLOGY
Centrifugation Comparative Study Culture Media Evaluation Studies
Hemolysis Human Mycobacterium avium Complex/GROWTH &
DEVELOPMENT/*ISOLATION & PURIF Mycobacterium avium-intracellulare
Infection/COMPLICATIONS/ DIAGNOSIS/MICROBIOLOGY Mycobacterium
tuberculosis/GROWTH & DEVELOPMENT/*ISOLATION & PURIF Support, Non-U.S.
Gov't Time Factors Tuberculosis,
Pulmonary/COMPLICATIONS/DIAGNOSIS/MICROBIOLOGY JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).